Table 1.
The effects of methimazole, heat treatment and cytochrome P450 reductase antiserum on the estimated kinetic parameters obtained for ABS production in human liver microsomes. Data are mean (±sd) for three livers. Significantly different from control valuea.
| Inhibitor | Km (μm) | Vmax (pmol min−1 mg−1) | CLint (μl min−1 mg−1) |
|---|---|---|---|
| Controls | 4.64±0.83 | 456.8±83.2 | 99.7±17.3 |
| Methimazole (25 μm) | 4.03±0.91 | 306.5±69.2a | 77.1±13.8 |
| Heat treatment (3 min at 45° C) | 3.72±2.18 | 288.7±69.0a | 93.7±45.6 |
| P450 oxidoreductase antiserum (30 μl) | 3.59±1.52 | 128.0±26.8a | 40.7±18.6a |