Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Simulation showing the effect of nonspecific binding with varying KD on Michaelis-Menten kinetics (a), and on an Eadie Hofstee plot (b). The plots use total (added) substrate concentration, whereas the reaction velocities were calculated using free (unbound) substrate concentrations.
