Figure 4.

Time course of the effects of N-CAM on stimulation of transcription via a GRE. Primary astrocytes were electroporated with the GRE-luc and CMV-β-gal vectors, cultured in serum-free medium for 48 hr, and then treated with 10 μg/ml N-CAM for 1–24 hr. Cell lysates were prepared and luciferase and β-gal activities were measured as described. The data are represented as a percentage of luciferase activity in control cells that were untreated. Each point represents the average of triplicate measurements with error bars showing the SD.