Table 1.
The effect of HU, MTX and FLU on 3TC phosphate levels and dCTP pools, in HepG2 cells incubated for 24 h.
| Drug | Total 3TC phosphates (pmol/106 cells) | Amount of 3TCTP (pmol/106 cells) | Amount of dCTP (pmol/106 cells) | 3TCTP/dCTP ratio |
|---|---|---|---|---|
| HU Control | 5.02 ± 0.47 | 0.79 ± 0.53 | 4.48 ± 1.61 | 0.176 |
| HU 10 μm | 8.51±6.14 (165) | 1.08 ± 0.56 (194) | 4.04 ±1.30 (92) | 0.267 |
| HU 100 μm | 12.22 ± 5.50 (239)** | 2.11±0.89 (361)** | 3.37 ± 1.36 (74)** | 0.626 |
| MTX Control | 5.66 ± 2.06 | 0.84 ± 0.34 | 4.03 ± 0.93 | 0.208 |
| MTX 0.1 μm | 10.56 ± 3.37 (196)* | 1.91 ± 1.19 (219)* | 5.27 ± 2.23 (128) | 0.362 |
| MTX 1 μm | 14.46 ± 5.81 (258)** | 2.45 ± 1.45 (267)** | 4.62 ± 1.68 (113) | 0.530 |
| FLU Control | 4.61 ± 0.19 | 1.11 ± 0.02 | 4.91 ± 0.47 | 0.226 |
| FLU 10 μm | 4.98 ± 0.84 (106) | 1.10 ± 0.29 (99) | 4.95 ± 0.45 (102) | 0.222 |
| FLU 100 μm | 9.03 ± 1.36 (193)*** | 1.72 ± 0.46 (155)* | 2.97 ± 0.55 (61)*** | 0.579 |
3TC concentration was 1 μm. Data expressed as mean ± s.d. (n = 4).% of control values in brackets. Data analysed by anova and modified t-test (Bonferroni).
*
P<0.05
**
P<0.01
***
P<0.001.