Table 2.
The effect of GCV, LCV and PCV on 3TC phosphate levels and dCTP pools, in HepG2 cells incubated for 24 h.
| Drug | Total 3TC phosphates (pmol/106 cells) | Amount of 3TCTP (pmol/106 cells) | Amount of dCTP (pmol/106 cells) | 3TCTP/dCTP ratio |
|---|---|---|---|---|
| GCV Control | 5.98 ± 1.35 | 0.52 ± 0.45 | 5.01±0.55 | 0.104 |
| GCV 1 μm | 5.95 ± 1.05 (102) | 0.52 ± 0.49 (96) | 5.04 ± 0.63 (101) | 0.103 |
| GCV 10 μm | 5.42 ± 1.27 (91) | 0.44 ± 0.39 (90) | 5.15 ± 0.36 (103) | 0.085 |
| LCV Control | 10.66 ± 1.54 | 2.31±0.51 | 4.23 ± 0.26 | 0.546 |
| LCV 1 μm | 10.42 ± 1.66 (98) | 2.50 ± 0.82 (107) | 4.06 ± 0.95 (95) | 0.616 |
| LCV 10 μm | 10.17 ± 2.02 (97) | 2.30 ± 1.05 (99) | 4.69 ± 0.21 (111) | 0.490 |
| PCV Control | 9.68 ± 0.27 | 1.62 ± 0.33 | 4.68 ± 0.20 | 0.346 |
| PCV 1 μm | 10.04 ± 0.61 (104) | 1.83 ± 0.37 (114) | 4.64 ± 0.68 (99) | 0.394 |
| PCV 10 μm | 9.68 ± 1.04 (100) | 1.85±0.43 (120) | 5.01 ± 1.01 (107) | 0.369 |
3TC concentration was 1 μm. Data expressed as mean ± s.d. (n = 4).% of control values in brackets. Data analysed by anova and modified t-test (Bonferroni).