Table 3.
The effect of ADV and FOS on 3TC phosphate levels and dCTP pools, in HepG2 cells incubated for 24 h.
| Drug | Total 3TC phosphates (pmol/106 cells) | Amount of 3TCTP (pmol/106 cells) | Amount of dCTP (pmol/106 cells) | 3TCTP/ACTP ratio |
|---|---|---|---|---|
| ADV Control | 6.93 ± 0.84 | 0.75 ± 0.13 | 2.67 ± 0.57 | 0.281 |
| ADV 1 μm | 6.67 ± 0.60 (97) | 0.83 ± 0.21 (111) | 2.78 ± 0.79 (105) | 0.299 |
| ADV 10 μm | 6.46 ± 0.81 (94) | 0.69 ± 0.08 (94) | 3.24 ± 1.12 (121) | 0.213 |
| FOS Control | 6.29 ± 0.98 | 1.35 ± 0.33 | 4.77 ± 0.25 | 0.283 |
| FOS 10 μm | 6.04 ± 0.66 (99) | 1.26 ± 0.53 (93) | 4.70 ± 0.88 (99) | 0.268 |
| FOS 100 μm | 5.74 ± 0.77 (94) | 1.21 ± 0.43 (88) | 4.57 ± 1.02 (96) | 0.265 |
3TC concentration was 1 μm. Data expressed as mean ± s.d. (n = 4).% of control values in brackets. Data analysed by anova and modified t-test (Bonferroni).