Figure 2.

Effect of antibodies raised against CYP1A2 (▪), 2B6 (•), 2C (▴) and 3A4 (♦) on the disappearance rate constant for propofol metabolism by human liver microsomes. The concentration of propofol was 20 µm. The amount of protein in liver microsomes in each incubation mixture was 100 µg. Microsomes and antibodies were preincubated at room temperature for 20 min, followed by addition of propofol and NADPH. The total amount of protein in the antibodies and preimmune immunoglobulin G was 50 µg. The results of duplicate determinations at each concentration of antibodies added to microsomes are shown. The mean (95% CI) rate constant for propofol metabolism in the absence of antibodies was 4.2 (2.7, 5.7) nmol min⁻¹ mg⁻¹ protein.