Figure 5.

Effects of uroguanylin^95–109 and uroguanylin^98–109 on cGMP accumulation and the affinities of uroguanylin^98–109 for binding to receptors on T84 cells at pH 5.0 versus pH 8.0. The data are representative experiments with duplicate assays. Each experiment was performed at least three times with similar results. The conditions are the same as those given in the Materials and Methods and the legends to Figs. 1 and 4. (Top) Stimulation of T84 cell cGMP accumulation by opossum uroguanylin^95–109. (Middle) Stimulation of cGMP accumulation by opossum uroguanylin^98–109. (Bottom) Inhibition of ¹²⁵I-ST binding to receptors on T84 cells by opossum uroguanylin^98–109.