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Zinc-induced activation of hTGFα production by 3T3 cells bearing a fusion gene (A) consisting of the hTGFα gene under the control of the mouse MT1 promoter (11). (B) Changes in hTGFα mRNA abundance in a transfected colony after treatment with zinc sulfate (100 μM), as determined by RNase protection assay. UP, undigested probe; DP, digested probe. (C) Release of TGFα protein to the culture medium in response to zinc by the colony depicted in B.
