Figure 4.
Increase in LEAC open probability (NpO) induced by fungal cell wall elicitor (n = 6), Pep-13 (n = 10), and structural derivatives of Pep-13 (n = 6, Pep-13A/12; n = 8, Pep-13/A2; n = 7, Pep-10, respectively). NpO was calculated from 60 s of recording obtained between 5 and 6 min after addition of elicitor as described in Materials and Methods and normalized to background activity recorded before addition of elicitor. Note the logarithmic scale used to plot increase in LEAC open probability. Data obtained from nonresponsive parsley protoplasts (□, see text for explanation) are included. Mean values ± SD, excluding those for nonresponsive protoplasts, are given. Elicitor concentrations used in patch-clamp experiments were 50 μg/ml fungal cell wall elicitor, 100 nM (Pep-13, Pep-13/A12), and 1 μM (Pep-13/A2, Pep-10), respectively. Amino acid sequences of Pep-13 and its structural derivatives are given in one-letter code. Underlined boldface letters represent alanine substitution sites within Pep-13.