Figure 2.

Inhibition of processing and endonuclease activities of ASV IN proteins by Y-3. (A) Processing activity of ASV IN (1–286) measured using Mn²⁺ (circles), Mg²⁺ (open diamonds), or Zn²⁺(triangles) as cofactor, with increasing concentrations of Y-3. Reactions containing Mn²⁺ were incubated at 37°C for 10 min.; those containing either Mg²⁺ or Zn²⁺ were incubated for 60 min. Processing activity is expressed as relative percentage of positive (no inhibitor) control. (B) Endonucleolytic activity of ASV IN (52–207) was measured using Mn²⁺(circles) or Zn²⁺(triangles), with increasing concentrations of Y-3. Reactions containing Mn²⁺ were incubated as above; those containing Zn²⁺ were incubated for 120 min. Data are expressed as in A.