Figure 2.

Chemical distribution of the protein and lipid characteristics of IR bands (amide II and CH₂ asymmetric stretching bands, respectively). (A) Optical image. (B) Protein distribution. (C) Lipid distribution. The mapping has been obtained by analyzing the intensity variation of a two-dimensional set of infrared spectra (14×14 points, step equal to 3 μm). Each spectrum was recorded in transmission through a 3×3-μm² aperture. The total recording time was 180 min. The spatial resolution is about between 2 and 3 μm.