Figure 2.

Analysis of LTD₄ metabolism by tissue homogenates of kidney, lung, and heart of wild-type (Left) and MBD-deficient (Right) mice by HPLC. The reaction conditions are as described under Materials and Methods. The peaks labeled as 1 and 2 refer to LTD₄ and LTE₄, respectively. For kidney (Top), 100 μg of protein and an incubation time of 30 min was used. Lung extracts were assayed using 50 μg of protein for 30 min (Middle). Heart extracts were incubated for 75 min and contained 100 μg of protein (Bottom).