Figure 4.

Divalent cation dependence of mAb CBRM1/20 binding to purified human neutrophil leukocytes. Neutrophils were washed with EDTA, then were washed and stained with mAb CBRM1/20 in the presence of the indicated divalent cation concentrations and were subjected to immunofluorescence flow cytometry. Mean fluorescence intensity is shown with intensity of X63 control staining subtracted. Fluorescence intensity is shown for mAbs CBRM1/32, CBRN1/6, and CBRN3/4 at 0 and 1 mM Ca²⁺.