Table 1.
Binding of mAb to Mac-1 α subunit mutants carrying human-to-mouse substitutions in the 1-2 loop of W5 and the 3-4 loop of W6*
| Mutation | CBRM 1/20 | CBRM 1/32 | CBRN 1/6 | CBRN 3/4 | CBRM 1/29 |
|---|---|---|---|---|---|
| Binding (% of human wild-type ± SD) | |||||
| hu(W5L1-2)mo | 0 ± 1 | 103 ± 9 | 101 ± 11 | 103 ± 12 | 104 ± 3 |
| V450M | 0 ± 2 | 87 ± 1 | 97 ± 2 | 86 ± 11 | 103 ± 4 |
| S452A | 107 ± 4 | 99 ± 5 | 93 ± 10 | 100 ± 2 | 91 ± 4 |
| N453D | 5 ± 3 | 101 ± 1 | 96 ± 2 | 99 ± 14 | 106 ± 0 |
| S455N | 93 ± 3 | 113 ± 8 | 108 ± 5 | 109 ± 12 | 107 ± 8 |
| D457N | 14 ± 4 | 70 ± 7 | 72 ± 11 | 71 ± 7 | 61 ± 7 |
| hu(W6L3-4)mo | 1 ± 1 | 104 ± 5 | 111 ± 6 | 106 ± 8 | 111 ± 3 |
| H541Y | 96 ± 12 | 97 ± 2 | 97 ± 9 | 106 ± 24 | 97 ± 4 |
| T543A | 104 ± 5 | 95 ± 2 | 100 ± 4 | 94 ± 0 | 90 ± 15 |
| G545I | 51 ± 1 | 95 ± 5 | 90 ± 1 | 95 ± 12 | 99 ± 5 |
| S546A | 92 ± 1 | 100 ± 1 | 98 ± 1 | 98 ± 2 | 100 ± 7 |
| G547S | 0 ± 1 | 92 ± 0 | 102 ± 14 | 84 ± 8 | 91 ± 1 |
| I548L | 108 ± 1 | 106 ± 7 | 93 ± 9 | 105 ± 2 | 106 ± 0 |
| P550A | 122 ± 6 | 97 ± 4 | 100 ± 18 | 83 ± 21 | 90 ± 16 |
| Q554H | 92 ± 1 | 102 ± 24 | 104 ± 14 | 116 ± 11 | 94 ± 19 |
*
COS-7 cells were cotransfected transiently with cDNAs for the mutated or wild-type Mac-1 α-subunit and human β2-subunit. Cells were stained with mAbs, and fluorescein isothiocyanate anti-IgG in L15 medium, which contains 1.26 mM Ca2+ and the % positive cells relative to wild-type, was determined after subtracting staining with X63 myeloma IgG.