Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1994 Sep;60(9):3458–3461. doi: 10.1128/aem.60.9.3458-3461.1994

Bioluminescent Most-Probable-Number Method To Enumerate lux-Marked Pseudomonas aeruginosa UG2Lr in Soil

C A Flemming 1, H Lee 1, J T Trevors 1,*
PMCID: PMC201832  PMID: 16349396

Abstract

Bioluminescence measured with a luminometer and charge-coupled device was an effective marker in most-probable-number assays for luxAB-marked Pseudomonas aeruginosa UG2Lr in soil. Most-probable-number assays with microtiter plate wells and luminometer tubes gave estimates for UG2Lr that were similar to viable colony counts. Both methods detected five cells per g of soil.

Full text

PDF
3458

Images in this article

3459Image
on p.3459

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COCHRAN W. G. Estimation of bacterial densities by means of the "most probable number". Biometrics. 1950 Jun;6(2):105–116. [PubMed] [Google Scholar]
  2. Dehority B. A., Tirabasso P. A., Grifo A. P., Jr Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium. Appl Environ Microbiol. 1989 Nov;55(11):2789–2792. doi: 10.1128/aem.55.11.2789-2792.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Flemming C. A., Leung K. T., Lee H., Trevors J. T., Greer C. W. Survival of lux-lac-marked biosurfactant-producing Pseudomonas aeruginosa UG2L in soil monitored by nonselective plating and PCR. Appl Environ Microbiol. 1994 May;60(5):1606–1613. doi: 10.1128/aem.60.5.1606-1613.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fredrickson J. K., Bezdicek D. F., Brockman F. J., Li S. W. Enumeration of Tn5 mutant bacteria in soil by using a most- probable-number-DNA hybridization procedure and antibiotic resistance. Appl Environ Microbiol. 1988 Feb;54(2):446–453. doi: 10.1128/aem.54.2.446-453.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Heitzer A., Webb O. F., Thonnard J. E., Sayler G. S. Specific and quantitative assessment of naphthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium. Appl Environ Microbiol. 1992 Jun;58(6):1839–1846. doi: 10.1128/aem.58.6.1839-1846.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hill P. J., Rees C. E., Winson M. K., Stewart G. S. The application of lux genes. Biotechnol Appl Biochem. 1993 Feb;17(Pt 1):3–14. [PubMed] [Google Scholar]
  7. Masson L., Comeau Y., Brousseau R., Samson R., Greer C. Construction and application of chromosomally integrated lac-lux gene markers to monitor the fate of a 2,4-dichlorophenoxyacetic acid-degrading bacterium in contaminated soils. Microb Releases. 1993 Mar;1(4):209–216. [PubMed] [Google Scholar]
  8. Masuko M., Hosoi S., Hayakawa T. A novel method for detection and counting of single bacteria in a wide field using an ultra-high-sensitivity TV camera without a microscope. FEMS Microbiol Lett. 1991 Jul 1;65(3):287–290. doi: 10.1016/0378-1097(91)90229-4. [DOI] [PubMed] [Google Scholar]
  9. Meighen E. A. Molecular biology of bacterial bioluminescence. Microbiol Rev. 1991 Mar;55(1):123–142. doi: 10.1128/mr.55.1.123-142.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Prosser J. I. Molecular marker systems for detection of genetically engineered micro-organisms in the environment. Microbiology. 1994 Jan;140(Pt 1):5–17. doi: 10.1099/13500872-140-1-5. [DOI] [PubMed] [Google Scholar]
  11. Rattray E. A., Prosser J. I., Killham K., Glover L. A. Luminescence-based nonextractive technique for in situ detection of Escherichia coli in soil. Appl Environ Microbiol. 1990 Nov;56(11):3368–3374. doi: 10.1128/aem.56.11.3368-3374.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rowe R., Todd R., Waide J. Microtechnique for most-probable-number analysis. Appl Environ Microbiol. 1977 Mar;33(3):675–680. doi: 10.1128/aem.33.3.675-680.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Russek E., Colwell R. R. Computation of most probable numbers. Appl Environ Microbiol. 1983 May;45(5):1646–1650. doi: 10.1128/aem.45.5.1646-1650.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Selifonova O., Burlage R., Barkay T. Bioluminescent sensors for detection of bioavailable Hg(II) in the environment. Appl Environ Microbiol. 1993 Sep;59(9):3083–3090. doi: 10.1128/aem.59.9.3083-3090.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shaw J. J., Dane F., Geiger D., Kloepper J. W. Use of bioluminescence for detection of genetically engineered microorganisms released into the environment. Appl Environ Microbiol. 1992 Jan;58(1):267–273. doi: 10.1128/aem.58.1.267-273.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Silcock D. J., Waterhouse R. N., Glover L. A., Prosser J. I., Killham K. Detection of a single genetically modified bacterial cell in soil by using charge coupled device-enhanced microscopy. Appl Environ Microbiol. 1992 Aug;58(8):2444–2448. doi: 10.1128/aem.58.8.2444-2448.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Dyke M. I., Couture P., Brauer M., Lee H., Trevors J. T. Pseudomonas aeruginosa UG2 rhamnolipid biosurfactants: structural characterization and their use in removing hydrophobic compounds from soil. Can J Microbiol. 1993 Nov;39(11):1071–1078. doi: 10.1139/m93-162. [DOI] [PubMed] [Google Scholar]
  18. Yu L. S., Fung D. Y. Five-tube most-probable-number method using the Fung-Yu tube for enumeration of Listeria monocytogenes in restructured meat products during refrigerated storage. Int J Food Microbiol. 1993 Apr;18(2):97–106. doi: 10.1016/0168-1605(93)90214-2. [DOI] [PubMed] [Google Scholar]
  19. de Weger L. A., Dunbar P., Mahafee W. F., Lugtenberg B. J., Sayler G. S. Use of Bioluminescence Markers To Detect Pseudomonas spp. in the Rhizosphere. Appl Environ Microbiol. 1991 Dec;57(12):3641–3644. doi: 10.1128/aem.57.12.3641-3644.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES