Figure 2.

Rho induces pol II release at all pause sites, including those induced by Reb1p. The template illustrated in Fig. 1 was transcribed by yeast pol II for 30 min. From each reaction, transcripts were then separated into a fraction still bound to the template (b) or a fraction released from the template (r). Reactions were run in either the absence of Reb1p (lanes 1–4) or the presence of saturating amounts of Reb1p (lanes 5–8). Rho protein was added to reaction in lanes 3,4 and 7,8 and was absent in lanes 1, 2, 5, and 6. The asterisk indicates a transcript 30 nucleotides shorter than those paused at the Reb1p binding site and that probably represents a second pol II ternary complex stacked up behind the complex paused at the Reb1p site.