Figure 6.

Rho-induced release of pol II requires ATP hydrolysis. pol II was used to transcribe the template illustrated in Fig. 1 in the presence of Reb1p but in the absence of rho protein for 10 min. Ternary complexes were then removed from the reaction, washed once, and resuspended for a further 20 min under the same reaction conditions plus the addition of rho protein and either ATP (lanes 1 and 2) or its nonhydrolyzable analog AMP-PNP (lanes 3 and 4). After the 20 min incubation transcripts were separated into either bound (b) or released (r) fractions.