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. 1998 Apr 28;95(9):4906–4911. doi: 10.1073/pnas.95.9.4906

Figure 2.

Figure 2

Detection of recombinant nematode EGases in E. coli lysates on Western blot (A) and CMC hydrolysis assays (B). (A) Immunodetection was done with subventral gland specific mAbs (17). Lanes: 1, HG-ENG-1 produced from pET28c; 2, pET28c control; 3, HG-ENG-2 produced from pET28a; 4, pET28a control; 5, GR-ENG-1 from vector pMAL-c2; 6, GR-ENG-2 from vector pMAL-c2; 7, pMAL-c2 control. (B) Detection of CMCase activity (halo) in affinity-purified heterologous cyst nematode EGases that correspond to lanes above.