Figure 4.

Localization of EGase transcripts and proteins within cyst nematodes. (A and B) Anterior sections of preparasitic J2 of H. glycines hybridized with digoxigenin-labeled RNA probes transcribed from the HG-eng-2 cDNA. (A) No staining is observed with the sense probe. (B) Specific binding of the antisense probe to the posterior region of the subventral gland cells (g). The gland extensions (e) remain unstained. n, nucleolus of dorsal gland; m, metacorpal pump chamber. (C and D) Immunofluorescence of H. glycines using antiserum raised against recombinant nematode HG-ENG2. (C) No specific labeling with the preimmune serum. (D) Intense labeling of subventral glands with HG-ENG-2-specific immune serum. (Bar = 20 μm.)