Table 1.

Partial amino acid sequences of purified nematode secretory proteins and deduced oligonucleotide primers used to amplify and clone EGase genes from G. rostochiensis and H. glycines

Nematode/primer*	Protein/DNA	Sequence
		Amino acid sequence†
H. glycines	svp49 (N terminal)	ASAVAPPFGQLSVSGSNLVGANKQPVQLISNSLFEL
G. rostochiensis	svp39 (N terminal)	LTATPPPYGQLSVSGTKLVGSMGQPVQLIGNSLFWPQFQGQYWNA
G. rostochiensis	svp39 (internal)	PTATYNLAVAVIEAAISQGMYVIVDTHSTEAHAD
		DNA sequence of deduced oligonucleotide primer‡
HG1f	svp49	5′-GCIGTIGCICCICCITTYGGICA-3′
GR1f	svp39 (N terminal)	5′-ACIGCIACICCICCICCITAYGGNCA-3′
GR2f		5′-CAGTGAAGGCRCTCAAATGCAATTGG-3′
GR3f		5′-CTTCCGTGTCTTCCTCCTCCATTG-3′
GR4f		5′-TGGGATCCACTGCCACGCCTCCCCCA-3′
HG2r		5′-ATAGCCGCCCTCGTCCAC-3′
GR5r	svp39 (internal)	5′-TGCCARTCIACDATNACRTACTKCC-3′
GR6r		5′-CCAATTGCATTTGAGYGCCTTCACTG-3′
GR7r		5′-GGAAGCTTGCGCAACCACTTTTTTATCATCATC-3′
GR8r		5′-GGAAGCTTCGCAACAATTTTATCGTCAATAAATT-3′
pCDNA5f	pCDNAII	5′-GGTGACACTATAGAATACTCAAGCTATGCA-3′
pCDNA3r	pCDNAII	5′-GACGGCCAGTGAATTGTAATACGACTCACT-3′

^*The forward (f) or reverse (r) orientation of the oligonucleotide primers is indicated in their names. 

^†The single character amino acid code is used. 

^‡I - Inosine, K = G + T, N = A + C + G + T, R = A + G, Y = C + T; underlining indicates endonuclease restriction site.