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. Author manuscript; available in PMC: 2008 Oct 2.
Published in final edited form as: FEBS Lett. 2007 Aug 31;581(24):4596–4602. doi: 10.1016/j.febslet.2007.08.048

Fig. 1.

Fig. 1

SAMMA inhibits HIV transmission by DCs. (a) MDDCs (1.25–2×105 per well) were exposed to replication-competent R5 virus HIVBaL at MOI of 0.002 in the presence or absence of SAMMA at 100 μg/ml at 37°C for 2 hr. Cells were washed and co-cultured with autologus activated primary CD4+ T cells (1×106) in complete media in the presence or absence of SAMMA at 100 μg/ml. (b) HIVBaL was pre-incubated without (control) or with SAMMA at 100 μg/ml at 37°C for 1 hr followed by addition to DCs (2×105) for 2 hr in the absence or presence of the inhibitor. After washing four times with media, DCs (1.25 × 105) were incubated with activated primary CD4+ T cells (1 × 106) in the absence of SAMMA. HIV released into media was monitored by measuring the level of HIV p24 antigen at day 0, 5, 7, 9, 14 after infection. Data in Fig. 1a are mean ± SD of duplicated samples and represent two independent experiments. In the wash-off setting (panel b), results from two different donors are shown.