Fig. 3.

SAMMA does not cause cytotoxicity in DCs and primary CD4+ T cells. DCs or activated primary CD4+ T cells (5×104) were treated with SAMMA at 10, 100, 500 μg/ml for three days at 37°C. Cytotoxicity was determined by measuring the number of viable cells by the MTS assay (Promega). MTS substrate was added to cells for 1 hr at 37°C. Data are mean ± SD of triplicated samples and represent two independent experiments.