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. 2007 Aug 8;35(16):5323–5337. doi: 10.1093/nar/gkm527

Figure 3.

Figure 3.

Different effects may relate to different levels of Cdc18 overexpression. Wild type (rad3+) cells, wild type (rad3+) cells containing pRep3X cdc18, and rad3ts cells containing pRep4X cdc18, were cultured for 24 h in the presence (OFF) or in the absence (ON) of thiamine. Wild type (rad3+) and rad3ts cdc18-T6A cells were also cultured for 24 h. Total protein lysates were separated on 10% SDS-PAGE and blotted on nitrocellulose membrane. The membrane was probed with polyclonal anti-Cdc18 antibodies (32). Note that the Cdc18-T6A band is higher in the gel than wild-type Cdc18 due to presence of the TAP tag. Note all lanes are from the same gel, separated to distinguish the slower running TAP-tagged Cdc18-T6A from wild Cdc18.