Table 1.

Apparent binding constants K_D and rate constants k of thiM TPP-riboswitch variants

Riboswitch variant	[nM]a	k25°C [M−1s−1]b
A69AP thiM 82	325	14.2 ± 0.45 × 104
G72AP thiM 82	265	12.1 ± 0.73 × 104
U79AP thiM 82	375	12.0 ± 0.42 × 104
U62AP thiM 82	320	9.26 ± 0.16 × 104
U46AP thiM 82	250	7.53 ± 0.23 × 104
A53AP thiM 82	435	3.85 ± 0.14 × 104
A85AP thiM 82	260	2.30 ± 0.20 × 104
U62AP thiM 81	495	8.66 ± 0.14 × 104
U62AP thiM 151	420	8.13 ± 0.16 × 104

^aValues are arithmetic means, determined from at least two independent titration experiments. ^bValues determined from at least three independent stopped-flow measurements.