Figure 3.

Spacer library sequences. The sequences of spacers for clones from the first and second library template oligonucleotides are shown in descending order of percent in vitro recombination. Clones from the second library template oligonucleotide are given below the dotted line. Spacer sequences for the lox sites 5′ to the kanamycin resistance marker and 3′ of the kanamycin marker are given. For clones with matching lox spacers only the 5′ site sequence is shown. For clones with unmatched spacers the sequence of both sites is shown. Spacers containing only 7 bases are marked with asterisks (*). Spacer sequences that appear more than once are underlined. The type of recombination products observed for in vivo reactions is noted in the middle column. All clones displayed either full recombination (F), a mixture of parental and recombination products (M) or no recombination products (N). The average percent recombination for in vitro Cre reactions is shown for each clone. The average and SD were calculated from three separate in vitro experiments.