Table 1.
Primers
Sequence | |
---|---|
shRNA primer | |
pSUPER-β-catenin | 5′GATCCCGTGGGTGGTATAGAGGCTC TTCAAGAGAGAGCCTCTATACCACC CACTTTTTGGAAA-3′ |
5′AGCTTTTCCAAAAAGTGGGTGGTAT AGAGGCTCTCTCTTGAAGAGCCTCT ATACCACCCACGG-3′ | |
pSUPER-p5O | 5′GATCCCTATTAGAGCAACCTAAACA TTCAAGAGATGTTTAGGTTGCTCT AATATTTTTGGAAA-3′ |
5′AGCTTTTCCAAAAATATTAGAGCA ACCTAAACATCTCTTGAATGTTTA GGTTGCTCTAATAGG-3′ | |
RT-PCR primer | |
Forward | 5′-CCTATGTATCCCTCAAAGCA-3′ |
Reverse | 5′-CCCACAGTGTATCCCTTCTT-3′ |
Luciferase primer | |
12-1 F | 5′-TACTCTCATTCTATCATTATTCCAA-3′ |
12-2 F | 3′-ATAACCTCTTTACGCCTCAGT-3′ |
12-2 R | 5′-CTTCTATGCTTACAAATGGTTATC-3′ |
3C-CRP primers | |
Upstream anchor | 5′-CTGTCCCACCACTCTCTATCTGA-3′ |
Fragment 1 | 5′-GACACATGAATAGTGGCTTTGCTC-3′ |
Fragment 2 | 5′-CATCGCAAAGAGGCTAAACTC-3′ |
Fragment 3 | 5′-GGGATATAATCTCAATCTCCTGGTG-3′ |
Fragment 4 | 5′-AGCAAAAGAGCAAAGGGAGA-3′ |
Fragment 5 | 5′-CTGATGCCTACTATAATGGATG TATTTC-3′ |
Fragment 6 | 5′-ACCCAGTCCAGATTAGGTCAGG-3′ |
Fragment 7 | 5′-GGCTATCAACATTCATCTCCTCAT-3′ |
Downstream anchor | |
Fragment 8 | 5′-CTTAAATTCTATACGTAAGTGAGGGG AT-3′ |
Fragment 9 | 5′-CGGTCCAAAAGAATCAGAATTT GA-3′ |
Fragment 10 | 5′-GTGGAGTCTCTGAATCAGCCTTG-3′ |
Fragment 11 | 5′-GATTGACAAACAACAGAGCCTTG AC-3′ |
Fragment 12 | 5′-CTGGTCTCTAAACATGGAGTTTT CC-3′ |
Fragment 13 | 5′-AGATCCCCCATGAGTGATTTAG TG-3′ |
Fragment 14 | 5′-CCCTCTTCACTAGGTATGAAGCA AG-3′ |
Fragment 15 | 5′-CACTATCTGAGGTCATCTGAGG AG-3′ |
Fragment 16 | 5′-GCTTCTGGGAATAAAGCAGGC-3′ |
Fragment 17 | 5′-CAACAGGGAAATCCTCGAATATG-3′ |
3C-GAPDH primers | |
GAPDH 1 | 5′-GTTTCCATAGGACCTGCTGCG-3′ |
GAPDH 2 | 5′-GTTTTACACTGG GCACTTGAG GTC-3′ |
ChIP-CRP primers | |
Fragment 4 Forward | 5′-TCTCCCTTTGCTCTTTTGCT-3′ |
Fragment 4 Reverse | 5′-CAATAAGTTCCAATGAAATA GACA-3′ |
Fragment 12 Forward | 5′-ATAACCTCTTTACGCCTCAGT-3′ |
Fragment 12 Reverse | 5′-CTTCTATGCTTACAAATGGTTATC-3′ |
Fragment 13 Forward | 5′-GGCTGATGGACTTAGGTAATACT-3′ |
Fragment 13 Reverse | 5′-TGACCAAACTGAGCGAACTC-3′ |