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. 2007 Aug 17;35(16):5593–5609. doi: 10.1093/nar/gkm598

Table 2.

Mathematical analysis of the segregation of tDNA sequences into Class I-II groups

Class I Class II


Number of false-positive sequencesa Probability (p) according to the statistical testb Number of false-positive sequencesa Probability (P) according to the statistical testb




Number of sequences SCPc ECPd SCPc ECPd Number of sequences SCPc ECPd SCPc ECPd
Saccharomyces cerevisiae 27 24 3 0.17 0.34 24 26 2 1.00 0.21
Schizosaccharomyces pombae 27 29 5 1.00 0.36 30 26 10 0.11 0.81
Caenorhabditis elegans 56 46 10 0.36 0.44 60 56 18 0.78 0.86
Drosophila melanogaster 44 31 4 0.11 0.81 34 44 8 1.00 0.89
Homo sapiens 60 57 34 0.89 0.13 58 43 12 0.07 0.55
Encephalitozoon cuniculi 22 22 2 0.86 0.20 23 22 8 0.61 0.91
Arabidopsis thaliana 75 63 1 0.60 0.03 71 54 1 0.38 0.03
Methanopyrus kandleri 18 8 2 0.15 0.22 15 8 3 0.04 0.18
Pyrococcus abyssi 25 20 2 0.58 0.26 20 16 2 0.39 0.20
Pyrobaculum aerophilum 23 21 3 0.91 0.19 22 15 6 0.44 0.53
Aeropyrum pernix 25 19 6 0.51 0.43 20 21 12 1.00 0.91
Archaeoglobus fulgidus 25 19 3 0.50 0.77 20 16 4 0.64 0.86
Halobacterium sp. NRC-1 25 16 2 0.04 0.31 20 25 3 1.00 0.26
Sulfolobus solfataricus 23 17 3 0.66 0.48 22 12 1 0.23 0.17
Sulfolobus tokodaii 23 20 3 0.89 0.31 22 16 3 0.46 0.28
Thermoplasma acidophilum 25 18 3 0.49 0.54 20 15 1 0.37 0.13
Ferroplasma acidarmanus 24 16 4 0.60 0.80 20 14 0 0.54 0.05
Methanosarcina barkeri 27 18 1 0.04 0.13 21 22 3 0.79 0.23
Methanococcus jannaschii 17 11 0 0.28 0.20 16 13 4 0.55 0.95
Methanobacterium thermoautotrophicum 20 13 2 0.44 0.66 16 14 3 0.77 0.68
Treponema pallidum 25 19 3 0.49 0.65 19 19 0 0.90 0.02
Borrelia burgdorferi 18 12 2 0.42 0.89 14 13 1 0.81 0.52
Chlamydia trachomatis 18 16 5 0.90 0.91 18 12 0 0.43 0.10
Synechocystis 6803 19 21 3 1.00 0.67 21 7 2 0.06 0.53
Anabaena 19 23 5 1.00 0.73 23 8 4 0.05 0.71
Lactococcus lactis 20 14 6 0.57 0.94 18 9 1 0.09 0.20
Listeria monocytogenes 19 13 1 0.41 0.29 20 15 6 0.72 0.96
Bacillus subtilis 23 16 4 0.55 0.63 21 17 2 0.76 0.24
Aquifex aeolicus 19 21 1 1.00 0.36 21 12 0 0.18 0.15
Mycobacterium tuberculosis 22 22 5 0.86 0.87 22 22 2 0.86 0.50
Deinococcus radiodurans 21 18 4 0.62 0.51 23 16 8 0.33 0.93
Neisseria meningitidis 22 20 7 0.74 0.97 20 14 6 0.37 0.89
Pseudomonas aeruginosa 20 21 5 1.00 0.61 21 13 5 0.27 0.67
Buchnera sp. APS 16 13 4 0.31 0.57 15 9 0 0.03 0.17
Bacillus halodurans 21 13 1 0.56 0.28 17 16 3 0.80 0.48
Thermotoga maritima 23 21 5 0.82 0.94 22 22 0 0.98 0.19
Campylobacter jejuni 19 12 4 0.28 0.89 15 12 1 0.34 0.20
Vibrio cholerae 25 22 2 0.58 0.42 22 17 3 0.35 0.46
Clostridium perfringens 20 18 3 0.55 0.65 18 20 1 1.00 0.17
Helicobacter pylori 19 13 2 0.56 0.66 16 11 1 0.32 0.32
Ralstonia solanacearum 20 23 6 1.00 0.91 23 13 2 0.17 0.46
Mycoplasma genitalium 18 17 6 0.89 0.98 17 14 1 0.62 0.23
Mycoplasma pneumoniae 19 17 5 0.89 0.94 17 14 1 0.61 0.23
Ureaplasma urealyticum 16 11 3 0.52 0.93 13 13 0 0.90 0.27
Xylella fastidiosa 22 22 5 0.98 0.64 22 15 2 0.46 0.17
Haemophilus influenzae 19 18 6 0.77 0.98 18 14 2 0.36 0.51
Escherichia coli 22 21 6 0.75 0.81 21 16 5 0.35 0.68
Rickettsia prowazekii 16 15 2 0.76 0.79 15 12 1 0.68 0.48
Yersinia pestis 21 22 7 1.00 0.86 22 15 4 0.32 0.61
Sinorhyzobium meliloti 43 22 22 1.00 0.60 22 12 24 0.00 0.39

aSequences that based on an analysis could belong to both classes are named as false positives in the a priory class, to which they are not considered to belong to as explained in Figures 1 and 2.

bThe bootstrap test quantifies the probability (P) of obtaining the observed number of false positives by pure chance, e.g. when the two classes are randomly defined from the same input tRNA pool (see Methods section). Cases with P ⩽ 0.25 are considered to be significant and are highlighted as bold.

cThe strict consensus partition (SCP) analysis admits a sequence into a class if the sequence possesses all the elements that are strictly present in the given class.

dThe extended consensus partition (ECP) analysis, on the other hand, admits a sequence into a class if the sequence does not possess any of the elements that are strictly absent from the given class. The species are arranged in blocks in the following order: Eukarya (top) Archaea (middle) and Bacteria (bottom) section.