Table 2.
Class I | Class II | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Number of false-positive sequencesa | Probability (p) according to the statistical testb | Number of false-positive sequencesa | Probability (P) according to the statistical testb | |||||||
Number of sequences | SCPc | ECPd | SCPc | ECPd | Number of sequences | SCPc | ECPd | SCPc | ECPd | |
Saccharomyces cerevisiae | 27 | 24 | 3 | 0.17 | 0.34 | 24 | 26 | 2 | 1.00 | 0.21 |
Schizosaccharomyces pombae | 27 | 29 | 5 | 1.00 | 0.36 | 30 | 26 | 10 | 0.11 | 0.81 |
Caenorhabditis elegans | 56 | 46 | 10 | 0.36 | 0.44 | 60 | 56 | 18 | 0.78 | 0.86 |
Drosophila melanogaster | 44 | 31 | 4 | 0.11 | 0.81 | 34 | 44 | 8 | 1.00 | 0.89 |
Homo sapiens | 60 | 57 | 34 | 0.89 | 0.13 | 58 | 43 | 12 | 0.07 | 0.55 |
Encephalitozoon cuniculi | 22 | 22 | 2 | 0.86 | 0.20 | 23 | 22 | 8 | 0.61 | 0.91 |
Arabidopsis thaliana | 75 | 63 | 1 | 0.60 | 0.03 | 71 | 54 | 1 | 0.38 | 0.03 |
Methanopyrus kandleri | 18 | 8 | 2 | 0.15 | 0.22 | 15 | 8 | 3 | 0.04 | 0.18 |
Pyrococcus abyssi | 25 | 20 | 2 | 0.58 | 0.26 | 20 | 16 | 2 | 0.39 | 0.20 |
Pyrobaculum aerophilum | 23 | 21 | 3 | 0.91 | 0.19 | 22 | 15 | 6 | 0.44 | 0.53 |
Aeropyrum pernix | 25 | 19 | 6 | 0.51 | 0.43 | 20 | 21 | 12 | 1.00 | 0.91 |
Archaeoglobus fulgidus | 25 | 19 | 3 | 0.50 | 0.77 | 20 | 16 | 4 | 0.64 | 0.86 |
Halobacterium sp. NRC-1 | 25 | 16 | 2 | 0.04 | 0.31 | 20 | 25 | 3 | 1.00 | 0.26 |
Sulfolobus solfataricus | 23 | 17 | 3 | 0.66 | 0.48 | 22 | 12 | 1 | 0.23 | 0.17 |
Sulfolobus tokodaii | 23 | 20 | 3 | 0.89 | 0.31 | 22 | 16 | 3 | 0.46 | 0.28 |
Thermoplasma acidophilum | 25 | 18 | 3 | 0.49 | 0.54 | 20 | 15 | 1 | 0.37 | 0.13 |
Ferroplasma acidarmanus | 24 | 16 | 4 | 0.60 | 0.80 | 20 | 14 | 0 | 0.54 | 0.05 |
Methanosarcina barkeri | 27 | 18 | 1 | 0.04 | 0.13 | 21 | 22 | 3 | 0.79 | 0.23 |
Methanococcus jannaschii | 17 | 11 | 0 | 0.28 | 0.20 | 16 | 13 | 4 | 0.55 | 0.95 |
Methanobacterium thermoautotrophicum | 20 | 13 | 2 | 0.44 | 0.66 | 16 | 14 | 3 | 0.77 | 0.68 |
Treponema pallidum | 25 | 19 | 3 | 0.49 | 0.65 | 19 | 19 | 0 | 0.90 | 0.02 |
Borrelia burgdorferi | 18 | 12 | 2 | 0.42 | 0.89 | 14 | 13 | 1 | 0.81 | 0.52 |
Chlamydia trachomatis | 18 | 16 | 5 | 0.90 | 0.91 | 18 | 12 | 0 | 0.43 | 0.10 |
Synechocystis 6803 | 19 | 21 | 3 | 1.00 | 0.67 | 21 | 7 | 2 | 0.06 | 0.53 |
Anabaena | 19 | 23 | 5 | 1.00 | 0.73 | 23 | 8 | 4 | 0.05 | 0.71 |
Lactococcus lactis | 20 | 14 | 6 | 0.57 | 0.94 | 18 | 9 | 1 | 0.09 | 0.20 |
Listeria monocytogenes | 19 | 13 | 1 | 0.41 | 0.29 | 20 | 15 | 6 | 0.72 | 0.96 |
Bacillus subtilis | 23 | 16 | 4 | 0.55 | 0.63 | 21 | 17 | 2 | 0.76 | 0.24 |
Aquifex aeolicus | 19 | 21 | 1 | 1.00 | 0.36 | 21 | 12 | 0 | 0.18 | 0.15 |
Mycobacterium tuberculosis | 22 | 22 | 5 | 0.86 | 0.87 | 22 | 22 | 2 | 0.86 | 0.50 |
Deinococcus radiodurans | 21 | 18 | 4 | 0.62 | 0.51 | 23 | 16 | 8 | 0.33 | 0.93 |
Neisseria meningitidis | 22 | 20 | 7 | 0.74 | 0.97 | 20 | 14 | 6 | 0.37 | 0.89 |
Pseudomonas aeruginosa | 20 | 21 | 5 | 1.00 | 0.61 | 21 | 13 | 5 | 0.27 | 0.67 |
Buchnera sp. APS | 16 | 13 | 4 | 0.31 | 0.57 | 15 | 9 | 0 | 0.03 | 0.17 |
Bacillus halodurans | 21 | 13 | 1 | 0.56 | 0.28 | 17 | 16 | 3 | 0.80 | 0.48 |
Thermotoga maritima | 23 | 21 | 5 | 0.82 | 0.94 | 22 | 22 | 0 | 0.98 | 0.19 |
Campylobacter jejuni | 19 | 12 | 4 | 0.28 | 0.89 | 15 | 12 | 1 | 0.34 | 0.20 |
Vibrio cholerae | 25 | 22 | 2 | 0.58 | 0.42 | 22 | 17 | 3 | 0.35 | 0.46 |
Clostridium perfringens | 20 | 18 | 3 | 0.55 | 0.65 | 18 | 20 | 1 | 1.00 | 0.17 |
Helicobacter pylori | 19 | 13 | 2 | 0.56 | 0.66 | 16 | 11 | 1 | 0.32 | 0.32 |
Ralstonia solanacearum | 20 | 23 | 6 | 1.00 | 0.91 | 23 | 13 | 2 | 0.17 | 0.46 |
Mycoplasma genitalium | 18 | 17 | 6 | 0.89 | 0.98 | 17 | 14 | 1 | 0.62 | 0.23 |
Mycoplasma pneumoniae | 19 | 17 | 5 | 0.89 | 0.94 | 17 | 14 | 1 | 0.61 | 0.23 |
Ureaplasma urealyticum | 16 | 11 | 3 | 0.52 | 0.93 | 13 | 13 | 0 | 0.90 | 0.27 |
Xylella fastidiosa | 22 | 22 | 5 | 0.98 | 0.64 | 22 | 15 | 2 | 0.46 | 0.17 |
Haemophilus influenzae | 19 | 18 | 6 | 0.77 | 0.98 | 18 | 14 | 2 | 0.36 | 0.51 |
Escherichia coli | 22 | 21 | 6 | 0.75 | 0.81 | 21 | 16 | 5 | 0.35 | 0.68 |
Rickettsia prowazekii | 16 | 15 | 2 | 0.76 | 0.79 | 15 | 12 | 1 | 0.68 | 0.48 |
Yersinia pestis | 21 | 22 | 7 | 1.00 | 0.86 | 22 | 15 | 4 | 0.32 | 0.61 |
Sinorhyzobium meliloti | 43 | 22 | 22 | 1.00 | 0.60 | 22 | 12 | 24 | 0.00 | 0.39 |
aSequences that based on an analysis could belong to both classes are named as false positives in the a priory class, to which they are not considered to belong to as explained in Figures 1 and 2.
bThe bootstrap test quantifies the probability (P) of obtaining the observed number of false positives by pure chance, e.g. when the two classes are randomly defined from the same input tRNA pool (see Methods section). Cases with P ⩽ 0.25 are considered to be significant and are highlighted as bold.
cThe strict consensus partition (SCP) analysis admits a sequence into a class if the sequence possesses all the elements that are strictly present in the given class.
dThe extended consensus partition (ECP) analysis, on the other hand, admits a sequence into a class if the sequence does not possess any of the elements that are strictly absent from the given class. The species are arranged in blocks in the following order: Eukarya (top) Archaea (middle) and Bacteria (bottom) section.