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Partially double-stranded linear DNA probe design. (A) HP:QO duplex formation in the absence of targets leads to fluorescence quenching. HP consists of 20, 25, 31 or 43 bases while QO consists of 12, 14 or 16 bases. QO sequence is complementary to 5′ sequence of HP. (B) HP binding to target during PCR amplification restores fluorescent signal by replacing QO.
