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. 2007 Aug 9;35(16):e101. doi: 10.1093/nar/gkm551

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Mismatch tolerance for HP43:QO14 (1:4) in melting analysis (A) and real-time PCR (B). Melting experiments were performed with HP43:QO14 (1:4) in the absence (open triangle) or presence of target with 0 (closed square), 1 (open square), 2 (open diamond), 3 (open circle) or 4 (closed triangle) mismatches in probe-binding region. Positions of mismatches are as follows: 1 mis is 12th or 27th nucleotide; 2 mis are the 9th and 12th, or 12th and 27th, or 3rd and 12th or 9th and 27th nucleotides; 3 mis are the 12th, 24th and 25th nucleotides; 4 mis are the 12th, 21st, 24th and 25th nucleotides. All mismatch nucleotide positions start from the 5′ end of HP43.