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. 2007 Aug 7;35(16):5303–5311. doi: 10.1093/nar/gkm569

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Fox-1 represses the intron 9 splicing in vitro. (A) Western blotting of the nuclear extracts using anti-Flag and anti-U2AF antibodies. HeLa cell nuclear extracts were mixed with nuclear extracts of HEK293 cells transfected with pCS2-Flag vector alone (Mock) or pCS2 Flag-mFox-1 (Flag Fox-1). The positions of molecular size markers are shown on the right side of upper panel. In addition to the band at the expected size, an additional band was detected (asterisk). (B) In vitro splicing reaction of Ex9-10 + 3GCAUG (lanes 1–4) and Ex9-10ΔGCAUG (lanes 5–8) in Mock nuclear extracts (lanes 2, 3, 7 and 8) or nuclear extracts containing Flag-tagged mFox-1 (lanes 4, 5, 9 and 10) for indicated time above each lane. Pre-mRNAs and splicing products are indicated schematically on the right.