Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Aug 7;35(16):5303–5311. doi: 10.1093/nar/gkm569

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Fox-1 blocks formation of the pre-spliceosomal early (E) complex on intron 9 via binding to GCAUG element in intron 8. (A) Spliceosomal complex formation on the Ex9-10 + 3GCAUG (lanes 1–4) and the Ex9-10 ΔGCAUG (lanes 1, 2, 5 and 6) transcripts in Mock nuclear extracts (lanes 1 and 2) or nuclear extracts containing Flag-tagged mFox-1 (lanes 3, 4, 7 and 8) under normal splicing condition in the presence of ATP for the indicated time above each lane. Spliceosomal complexes were separated by 4% native polyacrylamide gels and position of each complex is indicated on the left. (B) Spliceosomal complex formation on the hF1γEx 9-10 transcripts in the absence of ATP. Transcripts of the Ex9-10 + 3GCAUG (lanes 1–4) and the Ex9-10 ΔGCAUG (lanes 5–8) were incubated in Mock nuclear extracts (lanes 1, 2, 5 and 6) or Fox-1 nuclear extracts (lanes 3, 4, 7 and 8) in the absence of ATP and separated on a 1.5% native agarose gel. Positions of pre-spliceosomal complexes E and H are indicated on the left.