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. 2007 Aug 7;35(16):5303–5311. doi: 10.1093/nar/gkm569

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Identification of the functional region of Fox-1 protein required for alternative exon skipping. (A) Diagrams of full-length and truncated mutants of mFox-1 proteins. (B) Transfection analyses of the hF1γ L mini-gene. The hF1γ L mini-gene (lanes 1–5) were co-expressed in CV-1 cells along with pCS2+MT vector (lane 1), mFox-1 (lane 2), ΔN (lane 3), ΔC2 (lane 4), and ΔC3 (lane 5). The splicing products were analyzed by RT-PCR. The positions of spliced products are indicated on the right. (C) Western blotting of cell extracts to detect Fox-1 truncated mutants of Fox-1 proteins: Each of the truncated mutants was expressed from the pCS2+MT vector using the anti-Myc antibody. In lane 5, additional slow migrating bands as well as an expected band for ΔC3 protein were observed.