Table 1.

Proofreading and active site switching rates determined under single and multiple turnover conditions

Reaction conditions	Proofreading rates (s−1)	Active site switching rates (s−1)
WT T4 DNA pol (Figure 5A)DNA: Figure 1A, single Removal of one nucleotide	145 ± 3	exo-to-pol: 526
WT T4 DNA pol (Figure 5B)DNA: Figure 1A, multiple Removal of one nucleotide	106 ± 10 (0.4) 11 ± 0.9 (0.6)	pol-to-exo: 11
W213S-DNA polDNA: Figure 1A, multiple Removal of one nucleotide	1.5 ± 0.1
WT T4 DNA pol (Figure 6)DNA: Figure 1D, single Removal of two nucleotides	55 ± 2
Removal of the first nucleotide	88.5	exo-to-pol/ pol-to- exo: 159
Removal of the second nucleotide	145

Details of the reactions and the calculation of reaction rates are described in the text.