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. 2007 Aug 13;35(16):5409–5421. doi: 10.1093/nar/gkm524

Figure 1.

Figure 1.

IRF2 5′UTR sequence mediate internal initiation of translation. (A) Nucleotide sequence of interferon regulatory factor 2 (IRF2) 5′ UTR RNA. (B) MFOLD predicted secondary structure of IRF2 5′UTR. (C) Schematic representation of the bicistronic plasmids used in transient transfections is indicated. (D) Bicistronic plasmids (1 μg) of pRΔEnullF or pRIRF2F or pRBipF were transiently transfected into HeLa cells. Twenty-four hours post-transfection, respective luciferase activities corresponding to Fluc (white bar) and RLuc (gray bar) were measured and shown separately as fold increase compared with that from control (pRΔEnullF) taken as 100%. Transfection efficiencies were normalized by co-transfecting with a β-galactosidase plasmid. The data mean ± SD from three independent experiments. (E) Average absolute values of RLuc and FLuc activities (in relative light units) of the above transient transfection experiments conducted in trplicate are presented in the table. (F) Schematic representation of hairpin containing bicistronic plasmids used in transient transfections is represented. (G) Bicistronic plasmids (1 μg) containing delta EMCV sequence upstream or downstream of RLuc as indicated, were transfected into HeLa cells. The FLuc (white bar) and RLuc (gray bar) activities from the delta EMCV containing plasmids are shown as fold increase or decrease with respect to the corresponding controls, taken as 100. The data mean ± SD from three independent experiments.