Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Aug 13;35(16):5409–5421. doi: 10.1093/nar/gkm524

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Effect of tunicamycin on the IRES activity of IRF2. (A) HeLa cells were transiently transfected with bicistronic plasmids pRIRF2F or pRnullF. Four hours post-transfection, tunicamycin (Calbiochem) was added at a concentration of 2.5 μg/ml. Luciferase activity was measured 14 h post-transfection. FLuc (white bar) and RLuc (gray bar) activities are shown as fold decrease with respect to corresponding control (without tunicamycin), taken as 100%. The data mean ± SD from three independent experiments. (B) HeLa cells were transiently co-transfected with 1 μg of monocistronic plasmids pCDIRF2FLuc or pCDRLuc. 4 h post-transfection, tunicamycin (Calbiochem) was added at a concentration of 2.5 μg/ml. Luciferase activity was measured 14 h post transfection. FLuc (white bars) and RLuc (gray bars) activities correspond to the activities of the respective reporter constructs (as indicated). FLuc and RLuc activities are shown as fold decrease with respect to corresponding control (without tunicamycin) taken as 100%. The data mean ± SD from three independent experiments.