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. 2007 Aug 9;35(16):5360–5369. doi: 10.1093/nar/gkm508

Figure 2.

Figure 2.

T7 transcription mediated by the sPa pairing. (A) Schemes of the experiments. (B) Gel electrophoresis of transcripts generated from templates containing one or two Pa or z bases with the natural NTPs (1 mM) and sTP (1 mM). Transcripts were labeled at their 5′-termini with [γ-32P]GTP. The relative yields of each transcript were determined by comparison to the yields of native transcripts from templates consisting of the natural bases, and each yield was averaged from 3 to 4 data sets. (C) 2D-TLC analysis of the labeled ribonucleoside 3′-phosphates obtained from the nuclease digestion of the transcripts (17-mer). The transcripts were internally labeled with [α-32P]UTP. The spots on the TLC were obtained from the 17-mer fragment transcribed from the template (N1N2N3 = PaAC or CAC) in the presence of 1 mM sTP.