Table 2.
Melting temperatures obtained from fluorescent and UV profiles of yeast tRNAPhe containing s at specific sites
| Tmf values obtained from fluorescent profiles (°C) | Tm values obtained from UV profiles (°C) | |||||
|---|---|---|---|---|---|---|
| 0 mM Mg2+ | 2 mM Mg2+ | 5 mM Mg2+ | 0 mM Mg2+ | 2 mM Mg2+ | 5 mM Mg2+ | |
| Original tRNA | – | – | – | 56.1 | 65.5 | 70.1 |
| tRNA 16s | n.c. | 58.0 | 64.0 | 55.6 | 64.6 | 69.1 |
| tRNA 17s | n.c. | 59.6 | 65.5 | 55.7 | 65.6 | 69.1 |
| tRNA 36s | 57.0 | 69.5 | 74.0 | 55.2 | 65.2 | 69.7 |
| tRNA 47s | n.c. | 61.1 | 66.0 | 55.3 | 65.2 | 68.8 |
| tRNA 57s | 54.5 | 63.6 | 68.5 | 55.8 | 65.1 | 69.6 |
| tRNA 59s | 58.1 | 67.1 | 71.5 | 55.8 | 66.4 | 70.3 |
Fluorescent and UV profiles were obtained using each tRNA transcript (1 μM) in 50 mM sodium cacodylate (pH 7.2) and 50 mM KCl with or without MgCl2. Each melting temperature was calculated by using the IgorPro software (WaveMetrics). n.c.: not calculated.