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. Author manuscript; available in PMC: 2008 Oct 12.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Aug 9;362(1):188–192. doi: 10.1016/j.bbrc.2007.07.176

Figure 4.

Figure 4.

Activation of the ER stress response in 3T3-L1 adipocytes increases expression of CHOP10, and represses expression of GLUT4 and C/EBPα. Total RNA was purified from 3T3-L1 adipocytes treated with 2.5 µg/ml tunicamycin (Tunic), 2.5 µM thapsigargin (Thaps), or vehicle (Control) for 6 hours. Quantitative real-time RT-PCR was used to quantitate CHOP10, GLUT4, and C/EBPα mRNA levels. mRNA levels are expressed relative to levels in vehicle-treated cells, after controlling for 18S RNA expression level. Results represent the mean of 4 independent experiments; error bars indicate one standard deviation. * P < 0.05 vs. control.