Table 2.
Comparative analysis of cytokines released by LPS-activated TGF-GM-DCs and TGF-M-DCs
| Cytokines, pg/mL |
||
|---|---|---|
| CM: mature (LPS-stimulated) TGF-GM-DCs | CM: mature (LPS-stimulated) TGF-M-DCs | |
| Significant decrease of TGF-M-DCs to TGF-GM-DCs | ||
| IL-1β | 86 ± 4 | 3 ± 3* |
| IL-6 | 50305 ± 1282 | 20661 ± 2251† |
| GM-CSF | 559 ± 67 | 40 ± 14* |
| VEGF | 103 ± 4 | 15 ± 15† |
| Significant increase when comparing TGF-M-DCs to TGF-GM-DCs: IL-10 | 195 ± 115 | 829 ± 251* |
| No significant change from TGF-GM-DCs to TGF-M-DCs | ||
| TGF-β | 41 ± 13 | 27 ± 3 |
| M-CSF | 13 ± 13 | 34 ± 34 |
| PDGF-BB | 242 ± 101 | 151 ± 151 |
| IL-2 | 12 ± 1 | 22 ± 13 |
| IL-3 | ND | ND |
| IL-12 | 40 ± 40 | ND |
| IL-17 | ND | ND |
Conditioned medium prepared from 5 × 105 cells/mL. Data represent average cytokine levels of triplicate ± SEM from 4 different donors.
ND indicates nondetectable in all 4 donor samples.
*
P < .05 when using 2-tailed t test.
†
P < .05 when using 1-tailed t test.