Table 3.
Effects of ERK inhibitor on cytokine production by TGF-M-DCs and TGF-GM-DCs
| ERK inhibitor |
||
|---|---|---|
| − | + | |
| Cytokines decreased by ERK inhibitor | ||
| CM: TGF-M-DCs, pg/mL | ||
| TGFβ | 41 ± 13 | 9 ± 9† |
| IL-6 | 20661 ± 2251 | 9757 ± 3613 |
| CM: TGF-GM-DCs, pg/Ml | ||
| TGFβ | 27 ± 3 | 0 ± 0* |
| IL-6 | 50305 ± 1282 | 23300 ± 5558† |
| IL-1β | 86 ± 4 | 12 ± 6* |
| SDF-1 α | 725 ± 265 | 176 ± 135 |
| Cytokines increased by ERK inhibitor | ||
| CM: TGF-M-DCs, pg/mL | ||
| GM-CSF | 40 ± 14 | 155 ± 67 |
| CM: TGF-GM-DCs, pg/mL | ||
| GM-CSF | 559 ± 67 | 720 ± 72* |
| Cytokines unchanged by ERK inhibitor | ||
| CM: TGF-M-DCs, pg/mL | ||
| VEGF | 15 ± 15 | 33 ± 2 |
| PDGF-BB | 151 ± 151 | 350 ± 60 |
| IL-1β | 3 ± 3 | 4 ± 4 |
| SDF-1α | 1141 ± 139 | 1358 ± 159 |
| CM: TGF-GM-DCs, pg/mL | ||
| VEGF | 103 ± 4 | 117 ± 23 |
| PDGF-BB | 242 ± 101 | 363 ± 64 |
Cytokine levels were detected on protein array slides in triplicate for each sample treated ± ERK inhibitor U0126. Data represent average cytokine levels from triplicate samples ± SEM from 2 donors.
*
P < .05 when using 2-tailed paired t test.
†
P < .05 when using 1-tailed paired t test.