Table 3.

Decreased expression of genes restricted to myeloid lineage in Cited2^−/− fetal liver and Lin⁻c-Kit⁺ cells at 13.5 dpc

Gene name	Microarray	Real-time PCR
	Fetal liver	Fetal liver	Lin−c-Kit+ cells
Pre-pro proteinase3	−2.99	−2.78 ± 0.11	−2.9 ± 0.35
Myeloperoxidase	−2.53	−2.14 ± 0.02	−4.28 ± 0.16
Neutrophil elastase	−3.276	−3.275 ± 0.46	−5.77 ± 0.47
Cathepsin G	−2.738	−2.015 ± 0.12	−3.47 ± 0.07
Eosinophil peroxidase	−12.931	−6 ± 1.47	−6.12 ± 0.22
Wnt5a	−9.413	−4.68 ± 1.39	ND

Microarray data collected from Cited2^−/− fetal liver (n=3) and Cited2^+/+ littermate controls (n=3) were compared, and real-time PCR was performed to verify the expression of differentially expressed genes. HPRT was used as an internal control for real-time PCR, and the expression of each gene was normalized to HPRT and compared between Cited2^−/− and Cited2^+/+ littermate controls. The table shows mean (± SD) fold changes in Cited2^−/− (n = 3-5) fetal liver at 14.5 dpc and Lin⁻c-Kit⁺ cells at 13.5 dpc after comparison with wild-type littermate controls (n = 3-5).

ND indicates not detected.