Table 4.

Decreased expression of genes essential for HSC function in Cited2^−/− Lin⁻c-Kit⁺ cells at 13.5 dpc

Gene name	Real-time PCR Lin−c-Kit+ cells
Bmi-1	-6.08 ± 0.81
GATA-1	ND
GATA2	-4.42 ± 0.36
GATA3	ND
LEF-1	-15.5 ± 0.67
Mcl-1	-4.13 ± 0.1
Notch1	-2.9 ± 0.25
BMP4	NC

Real-time PCR was performed to analyze an array of genes essential for HSC function. HPRT was used as an internal control for real-time PCR, and the expression of each gene was normalized to HPRT and compared between Cited2^−/− and wild-type littermate controls. The table shows mean (± SD) fold changes in Cited2^−/− (n = 3) Lin⁻c-Kit⁺ cells at 13.5 dpc after comparison with wild-type littermate controls (n = 3).

ND indicates not detected; NC, no change.