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TGFβ-induced FOXP3+ T cells produced IL-2 and IFNγ. Flow cytometric analyses of FOXP3, IFNγ, IL-2, and IL-10 on day 7 activated adult CD4 + CD25hi nTregs, adult TGFβ-treated CD45RA+ cells, and cord-blood TGFβ-treated CD45RA+ cells restimulated with PMA and ionomycin. Data are representative of 3 independent experiments. Numbers in each quadrant as in Figure 1.
