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Electrophoretic analysis of genotyping PCR products. Genomic tail DNAs were prepared as detailed in the methods sections. 20 ng of the same DNA preparations of the four genotypes (KO male: y/- ; WT male: y/+; Heterozygote female: +/- ; WT female: +/+) were submitted to the different PCR amplification protocols detailed in the methods section, before loading on a 1.5% agarose gel.
