Figure 5.

Plot of OD₆₂₀ vs. column fraction numbers, for cell-free culture supernatants of the exoK exsH strain cultivated in GMS, subjected to various treatments, and passed over a Biogel A5-m gel filtration column. Plots represent cultures from which cells were removed by centrifugation at day 4 before treatment (dashed line) or cultures that were treated at day 3 with cells still present (solid line). In both cases, treatments were conducted for 24 hr. In the latter case, cells were removed by centrifugation after the 24-hr treatment. Treatments: ExoK (20 μg/ml) (A), ExsH (20 μg/ml) (B), succinoglycan depolymerase of Cytophaga arvensicola (C), soluble protein from BL21(DE3)/pET5a (D), and water (E).