Table 3.
Animals | |
Species | Adult male WKY/NCrl BR rats |
Number | n = 5 for each group |
Particles | |
Material | Titanium dioxide |
Aerosol generation | Spark generator (Palas) in a pure argon plus 0.1% oxygen stream |
Count median diameter (nm) | 22 (SD 1.7) |
Exposure | Inhalation of aerosol for 1 h |
Fixation and tissue processing | |
Time point of fixation | 1 h or 24 h after particle exposure |
Fixation mode | Subsequent perfusion fixation with 2.5% buffered glutaraldehyde, 1% osmium tetroxide, 0.5% uranyl acetate |
Tissue sampling | Systematic uniform random sampling |
Material for light and electron microscopy | Semithin (toluidine staining) and ultrathin (uranyl acetate and lead citrate staining) sections |