Figure 1.

Induction of Th1 cytokine mRNA and protein in ABL and CBL. mRNA levels (A, B) were determined using real-time RT-PCR after 4 hrs of stimulation. Data are expressed as arbitrary units normalized to β-actin to correct for RNA quantity and integrity. The dotted line indicates the basal mRNA level of untreated cells. Protein levels (C, D) were determined in the supernatant by ELISA after 24 hrs of stimulation. Bars are mean ± standard error for 3 donors. Similar results were obtained in 3 independent experiments. Although there was a clear coinciding tendency in all experiments, individual differences between blood samples often reduced the significance of the differences between CB and AB. Iono – Ionomycin. *p < 0.05, **p < 0.01 ABL vs. CBL.