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NOD1 and NOD2 mRNA expression in pulmonary leucocytes obtained from patients with tuberculosis and controls. NOD1 and NOD2 mRNA expression, measured by real-time RT-PCR, is similar in pulmonary leucocytes in 15 patients with tuberculosis compared to 6 healthy controls. To permit comparison between individuals, absolute copy numbers of NOD1 and NOD2 mRNA were measured during RT-PCR, using cDNA standards, and expressed relative to 106 mRNA copies of a validated housekeeping gene, HuPO.
