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. 2007 Aug 16;7:96. doi: 10.1186/1471-2334-7-96

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Copyright © 2007 Lala et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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NOD1 and NOD2 mRNA expression in peripheral blood cells obtained from patients with tuberculosis, before and after anti-tuberculous treatment. NOD1 and NOD2 mRNA expression was analysed, by real-time RT-PCR, in peripheral blood cells isolated from patients with tuberculosis and matched controls. To permit comparison between individuals, absolute copy numbers of NOD2 (4A) and NOD1 (4B) mRNA were measured during RT-PCR, using cDNA standards, and expressed relative to 10⁶mRNA copies of a validated housekeeping gene HuPO. NOD2 but not NOD1 mRNA levels increased significantly after treatment.